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Glycocarbohydrates detected inmilk-clotting proteases from Balanites aegyptiaca fruits using labeled lectins and gas chromatography

Robert Germain Beka, Valentin Desire Guiama, Esther Ngah, Marie-Christine Slomianny, Dominique Vercaigne-Marko

Proteases from Balanites aegyptiaca fruits pulp are glycosylated. This work was carried out to identify their glycans linked. Proteases extract was prepared by soaking B. aegyptiaca fruits in 50 mM, sodium citrate buffer pH 3 and purified using high cation exchange chromatography. The glycocarbohydrates linked to proteases was detected using both lectins, and gas chromatography coupled with flame ionization detector. The results indicate that the detection of glycocarbohydrates by gas chromatography is better after acetone precipitation. Gas chromatography detection reveal arabinose, rhamnose, xylose, galactose, mannose, glucose and glucuronic acid. Mannose and fucose was detected by labeled lectins.