अमूर्त

HPTLC Method for the Estimation of Cilostazol in Bulk and Formulation

Jayesh V. Patel, C. N. Patel, P. U. Patel, Pankaj H. Prajapati, I. S. Anand and M. B. Patel

A high performance thin layer chromatography (HPTLC) method was developed and validated for quantitative determination of cilostazol. The analysis was performed using a mobile phase composed of acetate : toluene : methanol : ether (2 : 2 : 1 : 0.5 v/v). The calibration graphs were linear (correlation coefficient (r) = 0.997) in the studied concentration range of 100 to 800 ng/mL for cilostazole in HPTLC. Relative standard deviation of all the parameters for intraday and interday precision is 0.75 to 2 and the accuracy was greater than 98%. The limit of quantification of the drug was found to be 20.5 ng/spot and the limit of detection of the drug was found to be 6.765 ng/spot. So the proposed method is precise and accurate and can be applied directly and easily to the cilostazol.