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Liquid chromatographic determination of certain isoquinoline alkaloid by a cloud-point extraction method with diatomite bonding magnetite

Jun-Chen You, Xiao-Ye Nie, Hao Wu, Li-Ming Du, Ya-Fen Chang, Yun-Long Fu

A synthesis of magnetic Fe3O4-doped diatomite nanoparticles (MNPS) to capture an anionic surfactant (SDBS) was described this paper, Using the above surfactant as an extractant to concentrate three kinds of isoquinoline alkaloids in which measured human urine by HPLC. The method affords significant simplification of the conventional CPE procedures without requiring specialized apparatus, thereby alleviating the need for specific sample handling treatment such as centrifugation. Furthermore, compared with conventional sorbents, analytes can be extracted fromlarger sample volumes at lower extraction times without any filtration step. The lower limits of quantification were 0.3 ng/mL for all analytes. The extraction recoveries were 87.5%for coptisine, 92.1%for palmatine, and 95.2%for berberine on average. The validated method was used to study the pharmacokinetic profile of the three isoquinoline alkaloid in urine after oral administration of the tablets.