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Simultaneous determination of ten herbicides residues in tobacco samples by gel permeation chromatography-gas chromatography

Huiqing Sun, Yiqiang Li, Guangjun Xu, XiuguoWang, Jinli Xu, Xiao Zheng

In this study, gas chromatography coupled with gel permeation chromatography for analysis of the residues of 10 herbicides (atrazine, clomazone, acetochlor, metolachlor, butralin, pendimethalin, butachlor, flumetralin, oxyfluorfen, quizalofop-p-ehtyl) in tobacco samples was developed. The herbicide residues were extracted from the tobacco with 1%acetic acid in acetonitrile. The extracts were purified by gel permeation chromatography, and analyzed by gas chromatographywith quantification by the external standard method. The elution time for herbicide was 15– 30min inGel Permeation Chromatography. The relativeretention time was 24.3–41.0 min in Gas Chromatography. The average recovery range for the herbicide residues was 83.8–100.1%, the relative standard deviation range was 1.93–8.91% and the detection limit range was 0.001–0.004 mg/ kg. Because this method is simple, accurate, has high sensitivity, and provides good purification, it is suitable for analysis of the herbicide residues in tobacco.