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Stability-indicating HPLC method for the determination of artesunate. Application to tablets

Amina Abdelal, Nahed El-Enany, Fathalla Belal

Asimple, stability-indicating, reversed phase high performance liquid chromatographicmethod has been developed for the determination of artesunate (ART) in presence of two of its alkaline degradation products. The analysis was performed using aNucleosil C18 column (150 x 4.6mmi.d.) as a stationary phase at ambient temperature with UV- detection at 215 nm. Amobile phase consisting of ofmethanol: acetonitrile: 0.05Mpotassiumdihydrogen phosphate solution of pH 3.9 (50: 8: 42, v/ v) was pumped at a flowrate of 2 mL min-1. The method showed good linearity over the range of 25-200 g mL-1with detection limit of 1.41 gmL-1and quantification limit of 4.28 gmL- 1 respectively. The proposed method was applied for the analysis ofART in pure form and commercial tablets with the mean % recoveries of 100.26 ± 1.34 and 100.30 ± 1.52 respectively. The results obtained were favorably compared to those obtained by the reference method.  2010 Trade Science Inc. - INDIA