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TLC-spectrodensitometric and microemulsion RP-HPLC chromatographic methods for determination of orphenadrine and paracetamol

Nouruddin W.Ali, Mohammed R.Elghobashy, Mohammed Gamal, Mohammed Abdelkawy

Two chromatographic methods were developed for determination of paracetamol and orphenadrine citrate in presence of p-aminophenol. In the first method, paracetamol, orphenadrine Citrate and p-aminophenol were separated on TLC silica gel 60F254 plate using ethyl acetate: acetone:methanol: glacial acetic acid (5: 4: 1: 0.1, by volume) asmobile phase. The obtained bands were then scanned at 220nm. The second method is a RP-HPLC method inwhich paracetamol, orphenadrine citrate and p-aminophenolwere separated on a reversed-phase C18 column using micro-emulsion as mobile phase at a flowrate of 1mL.min-1 andUV detection at 225nm. The proposed methods were successfully applied for determination of paracetamol and orphenadrine Citrate in pure formand in their pharmaceutical formulation.