अमूर्त

Validated RP-HPLC method for estimation of L-dopa from bulk and formulations

D.S.Ghodke, Nilofar S.Naikwade


L-dopa is a naturally occurring amino acid L-3,4-dihydroxyphenylalanine, is most efficacious drug in treatment of Parkinson’s disease. Various methods for analysis of the same are available but are time consuming and expensive. Here we have developed a new, precise and simple RP-HPLC method for estimation of L-dopa from bulk and formulation using a UV detector. The selected mobile phase was composed of 95:5 v/v of 25 mM ammoniumacetate and methanol pH 4.0. The wavelength selected was 280 nm. Thismethod was validated according to ICH Guidelines. There was no significant difference in the intraday and interday analysis of L-dopa determined for three different concentrations using this method. Linearity of the method was found to be 0-30g/ml with regression coefficient of 0.99921. The detection limit and quantitation limitwere 0.0024g/ml and 0.0073g/ml respectively. The method was found to be simple, accurate, precise, economical and robust.


में अनुक्रमित

  • कैस
  • गूगल ज्ञानी
  • जे गेट खोलो
  • चीन राष्ट्रीय ज्ञान अवसंरचना (सीएनकेआई)
  • उद्धरण कारक
  • ब्रह्मांड IF
  • इलेक्ट्रॉनिक जर्नल्स लाइब्रेरी
  • रिसर्च जर्नल इंडेक्सिंग की निर्देशिका (डीआरजेआई)
  • गुप्त खोज इंजन लैब्स
  • आईसीएमजेई

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